Childhood Adenotonsillectomy Trial

4.3.2 Daily Preparation

The following items should be on hand before beginning processing:

• Lab coats and gloves, splash shields, other Personal Protective Equipment, as needed
• Regridgerated Centrifuge: 2,000 g-force minimum, 4°C,and Swinging Bucket
• 10% bleach solution
• Test tube holders (adapters) for centrifuges
• Harvard Trip Balance / Pan balance
• Water bottles for balance
• Freezer (-70°C or colder or -20°C and dry ice)
• Emergency eye wash station
• Biohazard trash can, with large and small biohazard bags (bihazardous waste puncture-proof containers)
• Test tube racks / cryovial racks
• Fixed volume pipettes with tips (MLA) or adjustable pipettes (Rainin, Finn, etc) with tips. Volumes needed to pipette: 1.0 mL and 5.0 mL
• Cryovial and freezer box labels (provided by the LCBR)
• REVCO Freezer Boxes 5x5x2" and 5x5x3" and dividers (10x10 and 7x7 grids)
• Styrofoam/insulated shipping boxes
• Refrigerator - for storage of special blood tubes

Note: Can be a household fridge. Cannot be the same as a food fridge.

• Reagent bottles/containers for daily use
• Labels/lab tape for reagents
• Sharpie pens

Any partially-filled cryovial (less than the specified volume) should be marked with a dot on the cap and a "P" in the comment field on the Blood Processing Form next to that cryovial number.