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Childhood Adenotonsillectomy Trial

4.4.2.2 Procedure

For frozen shipment to the LCBR at the University of Vermont:

  1. Line Styrofoam mailer(s) with absorbent material (i.e. paper towels).
  2. Place approximately 1/2 the dry ice (per mailer) on the bottom of the mailer.
  3. Place another layer of absorbent material (i.e.: paper towels) on top of the dry ice so it will be between the dry ice and the freezer boxes containing the samples.
  4. Collect the freezer boxes containing samples to be shipped and check the sample ID numbers against the Processing Forms for that shipment.
  5. Place a rubber band around each cardboard freezer box containing samples before enclosing each box in a Ziploc plastic bag. Then carefully place these bagged boxes containing samples in the mailer. The rubber band is important for aiding in the prevention of a cryovial spill; the Ziploc bag and absorbent material are for compliance with commercial carrier specifications.
  6. Another layer of absorbent material is placed on top of the sample freezer boxes.
  7. The remaining dry ice is placed on top of this last layer of absorbent material. (Samples should not be on dry ice for > 24 hours).
  8. The top of the styro is sealed on with tape and then enclosed in the outer cardboard sleeve.
  9. The Processing and Collection Forms for all samples included in that particular Styrofoam mailers are placed on the top of the styro before the outer sleeve is securely taped closed.

Fill out the Shipping Log including the FedEx air bill numbers and fax to the LCBR at the University of Vermont at (802) 656-8965.

This shipping protocol follows the procedures mandated by the International Air Transport Association’s Dangerous Goods Regulations-Packaging Instructions 650 and 904.

NOTE: Only site personnel certified in Dangerous Goods Regulations-Packaging can package and ship biohazardous materials. Responsible site personnel must sign the CHAT Responsibility log and provide a copy of their Dangerous Goods certification to the project manager at the DCC.


National Sleep Research Resource
Childhood Adenotonsillectomy Trial