Cleveland Family Study

3.2 Equipment and Supplies

3.2.1 LCBR Recommended Supplies

• 5 mL SCAT-1 tubes (must be stored refrigerated until used): provided by the LCBR

• Cryovials – 0.5mL, 1.5 mL and 4.0 mL, and 10.0 mL with colored caps for color-coding:

  • Sarstedt 4.0 mL cryovial for Fletcher Allen Health Care tests: Catalog # 62.611
  • Krackler 10.0 mL cryovial for packed cells and urine samples: Catalog # 229-T310-10A

• Cap inserts for color coding:

  • Red (packed cells): Catalog # 229-T312-3
  • Green (urine + acid): Catalog # 229-T312-4
  • Yellow (plain urine): Catalog # 229-T312-5

• Fisher 0.5 and 1.5 cryovials and caps for blood samples:

  • 0.5 mL cryovials: Catalog # 02-681-333
  • 1.5 mL cryovials: Catalog # 02-681-338
  • Red caps: Catalog # 02-681-361
  • Orange caps: Catalog # 02-681-364
  • Blue caps: Catalog # 02-681-359
  • Yellow caps: Catalog # 02-681-360
  • White caps*: Catalog # 525-WF1S

*These caps are a special order item and may have to be ordered through the LCBR.

3.2.2 Blood Collection Supplies

• Lab coats and gloves
• Basin (just in case)
• Washcloths/Towels
• Smelling salts
• Plastic cart with wheels for phlebotomy supplies (or plastic tray with compartments)
• Butterfly needles (21 G) with luer adapter (Butterfly with safety needle 21G ¾ x 12”, B- D Vacutainer 367281)
• Vacutainer barrels
• Tourniquets
• Alcohol prep pads
• Gauze (2x2)
• Surgical tape or paper tape (easier on participants)
• Band-Aids
• Blood tube racks
• Scissors
• Pens
• Labels and Phlebotomy Form
• Biohazardous waste container
• Needle/sharps container
• Ice bucket and crushed ice - filled 10 min before draw
• Stopwatches or timers

• Blood collection tubes for PM, AM and OGTT draws (keep extras on hand):

  • (2) 10 mL Serum tubes (B-D 366430)
  • (2) 10 mL EDTA tubes (B-D 366457)
  • (2) 5 mL Citrate tubes (B-D 366415)
  • (2) 5 mL SCAT-1 tubes – provided by LCBR
  • (1) 5 mL Serum tube (B-D 366534)

• For Blind Duplicate Samples:

  • (1) 5 mL Serum (B-D 366534)
  • (1) 5 mL EDTA (B-D 366452)
  • (1) 5 mL Citrate (B-D 366415)
  • (1) 5 mL SCAT-1 – provided by LCBR

• Hemoguard caps on the Serum, EDTA and Citrate tubes can be used if desired.

3.2.3 Blood Processing Supplies

• Lab coats and gloves, splash shields, other Personal Protective Equipment as needed
• Lab mats and wipes
• Blood tube rocker
• 10% bleach solution or approved biohazard disinfectant
• Refrigerated centrifuge (4°C) capable of spinning at a minimum of 2000g
• -70°C freezer (or colder) or -20°C freezer and dry ice
• Refrigerator
• Harvard Trip Balance / Pan balance
• Water bottles for balance
• Test tube holders (adapters) for centrifuges
• Test tube racks / cryovial racks
• Fixed volume pipettes with tips (MLA) or adjustable pipettes with tips. Volumes needed to pipette: 0.5 mL, 1.0 mL and 3.0 mL
• Cryovial and freezer box labels – provided by the LCBR
• Revco Freezer Boxes 5x5x2" and 5x5x3" and dividers (10x10 and 7x7 grids)
• Styrofoam/insulated shipping boxes
• Reagent bottles/containers for daily use
• Labels/lab tape for reagents
• Sharpie pens
• Emergency eye wash station
• Biohazard trash can, with large and small biohazard bags (biohazardous waste puncture- proof containers)