Cleveland Family Study
3.6.7 Aliquoting Description and Scheme - 8:00 AM
8:00 AM Aliquot Assignments: * indicates samples for blind duplicates. The blind duplicate tube is collected after the regular tubes are filled. It would be the fifth tube filled at the 8:00 AM draw. The following blind duplicate tube types will be drawn:
- 5 mL EDTA (B-D 366452)
- 5 mL Serum (B-D 366534)
- 5 mL Citrate (B-D 366415)
- 5 mL SCAT-1
| Tube # | Tube Type | # Vials | Cap Color | Vial Size (mL) | Sample Vol. (mL) |
|---|---|---|---|---|---|
| 1 | Serum | 1 | Clear | 4.0 | 1.0 |
| 1 | Serum | 3 | Red | 1.5 | 1.0 |
| 2 | Citrate | 4 | Blue | 0.5 | 0.5 |
| 3 | EDTA | 1 | Clear | 4.0 | 1.0 |
| 3 | EDTA | 3 | White | 1.5 | 1.0 |
| 4 | SCAT-1 | 4 | Yellow | 0.5 | 0.5 |
| (5*) | Serum | 1 | Clear | 4.0 | 1.0 |
| (5*) | Serum | 2 | Red | 1.5 | 1.0 |
| (6*) | Citrate | 3 | Blue | 0.5 | 0.5 |
| (7*) | EDTA | 1 | Clear | 4.0 | 1.0 |
| (7*) | EDTA | 2 | White | 1.5 | 1.0 |
| (8*) | SCAT-1 | 3 | Yellow | 0.5 | 0.5 |
8:00 AM Aliquot Scheme:
| AM Vial# | Draw Tube | Vial Size (mL) | Color | Aliquot Vol. (mL) |
|---|---|---|---|---|
| 17 | Citrate | 0.5 | Blue | 0.5 |
| 18 | Citrate | 0.5 | Blue | 0.5 |
| 19 | Citrate | 0.5 | Blue | 0.5 |
| 20 | Citrate | 0.5 | Blue | 0.5 |
| 21 | EDTA | 4.0 | Clear | 1.0 |
| 22 | EDTA | 1.5 | White | 1.0 |
| 23 | EDTA | 1.5 | White | 1.0 |
| 24 | EDTA | 1.5 | White | 1.0 |
| 25 | SCAT-1 | 0.5 | Yellow | 0.5 |
| 26 | SCAT-1 | 0.5 | Yellow | 0.5 |
| 27 | SCAT-1 | 0.5 | Yellow | 0.5 |
| 28 | SCAT-1 | 0.5 | Yellow | 0.5 |
| 29 | Serum | 4.0 | Clear | 1.0 |
| 30 | Serum | 1.5 | Red | 1.0 |
| 31 | Serum | 1.5 | Red | 1.0 |
| 32 | Serum | 1.5 | Red | 1.0 |
| 33 | - | 10.0 | Red | 9.0 |
| (34*) | Serum | 4.0 | Clear | 1.0 |
| (35*) | Serum | 1.5 | Red | 1.0 |
| (36*) | Serum | 1.5 | Red | 1.0 |
| (37*) | Citrate | 0.5 | Blue | 0.5 |
| (38*) | Citrate | 0.5 | Blue | 0.5 |
| (39*) | Citrate | 0.5 | Blue | 0.5 |
| (40*) | EDTA | 4.0 | Clear | 1.0 |
| (41*) | EDTA | 15 | White | 1.0 |
| (42*) | EDTA | 15 | White | 1.0 |
| (43*) | SCAT-1 | 0.5 | Yellow | 0.5 |
| (44*) | SCAT-1 | 0.5 | Yellow | 0.5 |
| (45*) | SCAT-1 | 0.5 | Yellow | 0.5 |
- Citrate – After centrifugation, 0.5 mL of plasma is pipetted into cryovials #17–20. These will be blue-capped cryovials. The packed cells remaining in the tube can be added to tube #33. Tube #33 is a red-capped 10.0 mL tube.
- EDTA – After centrifugation, plasma will be pipetted into cryovials # 21–24. 1.0 mL of plasma is added to clear-capped vial #21. 1.0 mL of plasma is added to white-capped vials #22-24. The packed cells remaining in the tube are added to tube #33.
- SCAT-1 – After centrifugation, plasma will be pipetted into cryovials #25–28. These will be yellow-capped cryovials. The packed cells remaining in the tube are added to tube #33.
- Packed Cells – The packed cell tube #33 is a red-capped cryovial that will be frozen and shipped to the LCBR for storage.
Note: Packed cells are only collected from the 8:00 AM draw and not the PM draw. Although the tube holds 10 mL, only 9 mL of material can be added to the tube due to expansion during freezing. The cells from the EDTA and SCAT-1 tubes should be added to tube #33 first and, if there is sufficient space, cells from the citrate tube can also be added for a total volume of 9 mL
- Serum – 1.0 mL of the serum is pipetted into cryovials #29–32. Tube #29 is a clear-capped cryovial and tubes #30–32 are red-capped cryovials.
- Blind Duplicates* – The tubes are handled in the same way as the regular collection tubes. The EDTA, Citrate and SCAT-1 tubes are placed on the mixer for approximately 30 seconds, then placed in ice, and centrifuged within 15 to 30 minutes. The Serum tube remains at room temperature for a minimum of 40 minutes to clot before centrifuging.
Once the samples are aliquoted, the EDTA, Citrate and SCAT-1 blood collection tubes can be discarded in the biohazard waste (puncture-proof containers).