Cleveland Family Study

3.6.3 Centrifugation

Once centrifugation is complete, tubes are carefully placed on ice and are ready to aliquot.

3.6.3.1 EDTA, SCAT-1, and Citrate Tubes

Begin processing EDTA, SCAT-1, and Citrate tubes within 15 minutes of venipuncture. If centrifugation is not immediate, tubes are stored upright on wet ice for up to 30 minutes. Tubes are centrifuged at 4°C at a minimum of 2,000 x g for 15 minutes or 3,000 x g for 10 minutes (for a total of 30,000 g-minutes). Maximum time elapsed before centrifugation is 30 minutes from time of collection. Please note all start times on the Blood Processing Form. Once centrifugation is complete, tubes are carefully placed on ice and are ready to aliquot.

3.6.3.2 Serum

Allow the Serum tube to clot for at least 40 minutes at room temperature (maximum time before centrifugation is 90 minutes). These tubes are centrifuged at 4°C at 2,000g for 15 minutes or 3,000g for 10 minutes for a total of 30,000 g-minutes. After centrifugation is complete, place on ice.