Cleveland Family Study
3.6.6 Aliquoting Description and Scheme - 10:30 PM
10:30 PM Aliquot Assignments:
| Tube # | Tube Type | # Vials | Cap Color | Vial Size (mL) | Sample Vol. (mL) |
|---|---|---|---|---|---|
| 1 | Serum | 4 | Red | 1.5 | 1.0 |
| 2 | Citrate | 4 | Blue | 0.5 | 0.5 |
| 3 | EDTA | 4 | White | 1.5 | 1.0 |
| 4 | SCAT-1 | 4 | Yellow | 0.5 | 0.5 |
10:30 PM Aliquot Scheme:
| PM Vial# | Draw Tube | Vial Size (mL) | Color | Aliquot Vol. (mL) |
|---|---|---|---|---|
| 1 | Citrate | 0.5 | Blue | 0.5 |
| 2 | Citrate | 0.5 | Blue | 0.5 |
| 3 | Citrate | 0.5 | Blue | 0.5 |
| 4 | Citrate | 0.5 | Blue | 0.5 |
| 5 | EDTA | 1.5 | White | 1.0 |
| 6 | EDTA | 1.5 | White | 1.0 |
| 7 | EDTA | 1.5 | White | 1.0 |
| 8 | EDTA | 1.5 | White | 1.0 |
| 9 | SCAT-1 | 0.5 | Yellow | 0.5 |
| 10 | SCAT-1 | 0.5 | Yellow | 0.5 |
| 11 | SCAT-1 | 0.5 | Yellow | 0.5 |
| 12 | SCAT-1 | 0.5 | Yellow | 0.5 |
| 13 | Serum | 1.5 | Red | 1.0 |
| 14 | Serum | 1.5 | Red | 1.0 |
| 15 | Serum | 1.5 | Red | 1.0 |
| 16 | Serum | 1.5 | Red | 1.0 |
- Citrate – After centrifugation, carefully pipette 0.5 mL of this plasma into each cryovial #1–4. These will be blue-capped cryovials.
- EDTA – After centrifugation, 1.0 mL this plasma can now be aliquoted into cryovials #5–8. These will be white-capped cryovials.
- SCAT-1 – After centrifugation, carefully pipette 0.5mL of this plasma into each of the cryovials #9–12. These will be yellow-capped cryovials.
- Serum – Carefully pipette 1.0 mL of serum into each of the cryovials #13–16. These will be red capped cryovials. The remaining red cells in the serum draw tube (and the tube itself) can be discarded in the biohazardous waste.
Once the samples are aliquoted, the EDTA, Citrate and SCAT-1 blood collection tubes can be discarded in the biohazard waste (puncture-proof containers).