Heart Biomarker Evaluation in Apnea Treatment

4.1.2 Equipment and Supplies

The following supplies are recommended:

• 5 mL SCAT-1 tubes (must be stored refrigerated until used)
• Cryovials – 0.5mL, 1.5 mL and 4.0 mL, and 10.0 mL with colored caps for color-coding
• Sarstedt 4.0 mL cryovial (for clinical tests): Catalog # 62.611
• Krackler 10.0 mL cryovials for packed cells and urine samples: Catalog # 229-T310-10A (These are white caps that can be colored in with a red, green or yellow permanent marker instead of purchasing the below colored caps.)
  • Cap Inserts for Color Coding:
    • Red (packed cells): Catalog # T345-R
    • Green (urine + acid): Catalog # T345-G
    • Yellow (plain urine): Catalog # T345-Y
  • Fisher 0.5 and 1.5 Cryovials and caps for blood samples:
    • Red caps: Catalog # 02-681-361
    • Green caps: Catalog # 02-681-363
    • Blue caps: Catalog # 02-681-359
    • Yellow caps: Catalog # 02-681-360
    • Purple caps: Catalog # 02-681-366
    • Clear caps: Catalog # 02-681-358
    • 0.5 mL cryovials: Catalog # 02-681-333
    • 1.5 mL cryovials: Catalog # 02-681-338

Blood collection requires the following supplies:

• Lab coats and gloves
• Basin (just in case)
• Washcloths/Towels
• Smelling salts
• Plastic cart with wheels for phlebotomy supplies (or plastic tray with compartments)
• Butterfly needles (21 G) with luer adapter (Butterfly with safety needle 21G ¾ x 12", B-D Vacutainer 367281)
• Vacutainer barrels
• Tourniquets
• Alcohol prep pads
• Gauze (2x2)
• Surgical tape - paper tape (easier on participants)
• Band-Aids
• Blood tube racks
• Scissors
• Pens
• Labels and Phlebotomy Form*
• Biohazardous waste container
• Needle/sharps container
• Ice bucket and crushed ice - filled 10 min before draw
• Stopwatches or timers
• Blood collection tubes for AM draw (keep extras on hand):
  • 1 – 10 mL Serum tubes (B-D 367820)
  • 1 – 10 mL EDTA tubes (B-D 366457)
  • 1 – 5 mL Citrate tubes (B-D 366415)
  • 1 - 5 mL SCAT-1 tubes
  • 1 – 8mL CPT tube B-D 362753 (heparinized plasma) (see Appendix A for protocol and processing)
• Hemoguard caps on the Serum, EDTA and Citrate tubes can be used if desired.

The blood processing area should have the following additional supplies and equipment:

• Lab mats and wipes
• Blood tube rocker
• 10% bleach solution or approved biohazard disinfectant
• Refrigerated centrifuge (4°C) capable of spinning at a minimum of 2000 x g
• -70°C freezer (or colder) or -20°C freezer and dry ice
• Refrigerator

*Provided by the LCBR