Heart Biomarker Evaluation in Apnea Treatment

4.5.2.2 Procedure

For frozen shipment to the LCBR at the University of Vermont:

1. Line styrofoam mailer(s) with absorbent material (i.e. paper towels).
2. Place approximately 1/2 the dry ice (per mailer) on the bottom of the mailer.
3. Place another layer of absorbent material (i.e.: paper towels) on top of the dry ice so it will be between the dry ice and the freezer boxes containing the samples.
4. Collect the freezer boxes containing samples to be shipped and check the sample ID numbers against the Processing Forms for that shipment.
5. Place a rubber band around each cardboard freezer box containing samples before enclosing each box in a ziplock plastic bag. Then carefully place these bagged boxes containing samples in the mailer. The rubber band is important for aiding in the prevention of a cryovial spill; the ziplock bag and absorbent material are for compliance with commercial carrier specifications.
6. Another layer of absorbent material is placed on top of the sample freezer boxes.
7. The remaining dry ice is placed on top of this last layer of absorbent material.
8. The top of the styrofoam is sealed on with tape, and then enclosed in the outer cardboard sleeve.
9. The Processing and Collection Forms for all samples included in that particular styrofoam mailer, are placed on the top of the styrofoam before the outer sleeve is securely taped closed.
10. Affix shipping label(s). Place the entire box in the refrigerator if pickup is not immediate. (Samples should not be on dry ice for > 24 hours).
11. Affix shipping labels (Fed Ex label, Biological Specimen Category B UN3373 label, Dry Ice Class 9 UN1845 label, and Keep Frozen label) to outside of shipping container.

Fill out the Shipping Log including the FedEx airbill numbers and fax to the LCBR at the University of Vermont.

This shipping protocol follows the procedures mandated by the International Air Transport Association’s Dangerous Goods Regulations-Packaging Instructions 650 and 904.