Heart Biomarker Evaluation in Apnea Treatment

4.4.5 Urine Aliquoting

1. Pipette two 9.0 mL samples of urine from container 1 into 10.0 mL yellow-capped tubes # 22 - 23 and place on ice.
2. Pipette one 9.0 mL samples of urine from container 2 (urine + acid) into 10.0 mL green-capped tube # 24 and place on ice.
3. Freeze the samples at -70°C or on dry ice within 10 minutes of aliquot preparation.
4. Discard left-over urine.
5. Check-off on Processing Form the number of urine aliquots made.
6. The completed Urine Collection/Processing Form is sent to the LCBR.

Aliquot Assignments

NOTE: DO NOT OVERFILL the urine aliquots. There needs to be adequate space for the urine to expand when frozen without displacing the cryovial cap.