If centrifugation is not immediate, tubes are stored upright on wet ice for up to 30 minutes. Tubes are centrifuged at 4°C at a minimum of 2,000 x g for 15 minutes or 3,000 x g for 10 minutes (for a total of 30,000 g-minutes). Maximum time elapsed before centrifugation is 30 minutes from time of collection. Please note all start times on the Blood Processing Form. Once centrifugation is complete, tubes are carefully placed on ice and are ready to aliquot.